ELISA oo soo gaabisay waxay u taagan tahay "imtixaanka difaaca jirka ee ku xiran enzyme." Waa farsamo bayochemical ah oo loo isticmaalo shaybaarrada lagu ogaado laguna qiyaaso joogitaanka walxaha gaarka ah, sida antigens ama unugyada difaaca jirka, ee muunad. ELISA waxaa caadi ahaan loo adeegsadaa baarista caafimaadka, cilmi baarista, iyo qeybaha kale ee sayniska. Mabda'a aasaasiga ah ee ELISA waxay ku lug leedahay xidhitaanka molecule bartilmaameedka ah (tusaale, antigen ama antibody) dusha adag, oo ay ku xigto isticmaalka molecules-ku-xidhan enzyme-ka iyo substrate-ka midabaysan ama fluorescent si loo ogaado joogitaanka molecule bartilmaameedka. ELISA waa imtixaan aad loo isticmaalo oo la wada isticmaalo oo leh codsiyo dhinacyo kala duwan oo bayooloji ah, oo ay ku jiraan immunology, biology molecular, and clinical diagnostics.