Novobiocin suna ne da ke nufin maganin rigakafi wanda aka samo daga nau'in ƙwayoyin cuta na Streptomyces. Ana amfani da ita sosai wajen magance cututtukan ƙwayoyin cuta, musamman waɗanda staphylococci ke haifar da su da sauran ƙwayoyin cuta masu gram-positive. Novobiocin yana aiki ta hanyar hana ayyukan DNA gyrase, wani enzyme wanda ke da mahimmanci don kwafin DNA na kwayan cuta. A wasu lokuta ana amfani da maganin tare da sauran ƙwayoyin cuta don ƙara tasirinsa akan wasu nau'ikan cututtuka.